Fluorescence Filter

1 Features

• High Transmission: The Fluorescence Filter ensures high transmission for specific narrow wavelength ranges, allowing for brighter and clearer fluorescence images.
• Sharp Slope: Designed with a sharp slope, this filter isolates narrow wavelengths with high precision, improving the signal-to-noise ratio in fluorescence imaging.
• Wide Application Range: Suitable for single and multiple microscopy applications, including GFP FRET, photons, quantum dots, and contrast imaging.
• Deep Blocking: Effectively blocks unwanted wavelengths, ensuring no stray light reaches the detector, which guarantees better image quality.
• Durable and Reliable: Constructed with high-quality optical materials, these filters are durable, stable, and provide consistent performance in demanding conditions.

2. General Description

Shape Optics Technologies Pte Ltd (SOT) offers Fluorescence Filters (Narrow Bandpass Filters) designed to isolate and transmit a specified narrow wavelength range while reflecting all other incident wavelengths. These filters are essential for applications such as fluorescence microscopy, biotechnology, clinical chemistry, and other analytical techniques that require the precise isolation of light.

The high transmission of SOT’s fluorescence filters ensures that the fluorescence signal is bright and clear. The narrow bandpass ensures that only the relevant wavelengths pass through, while the deep blocking feature prevents other unwanted light from reaching the detector. This makes them ideal for use in applications such as GFP FRET, photon detection, quantum dot imaging, and contrast imaging.

These filters are designed for use in various microscope systems, fluorescence analyzers, and other optical instruments requiring high precision and sharp wavelength isolation. Available in multiple sizes (Φ8mm, Φ12mm), SOT’s fluorescence filters are fabricated to meet the highest standards, offering excellent performance in both single and multi-wavelength applications.

3. Spectrum Transmission Curves

If the standard components are not suitable for your application, SOT offers customized solutions. Custom fluorescence filters can be tailored to your exact wavelength and transmission specifications. Contact us for more information and to request a quote.

4. Coating Quality

• Adhesion Test: The coatings used on the filters comply with MIL-C-675C military standards. The adhesion test involved applying a tape that meets LT-90 specifications to the lens film layer. The tape was affixed fully and removed vertically three times, resulting in no blistering or peeling.
• Temperature Test: Following MIL-C-675C specifications, test pieces were subjected to temperatures of -62±1℃ and 71±1℃. After holding the pieces at room temperature (16℃–32℃) for two hours, the adhesion test was repeated and confirmed that the film remained intact with no detachment.
• Abrasion Resistance Test: The filter passed the abrasion resistance test according to MIL-C-675C and CCC-C-440 standards, using a gauze tester that applied a minimum force of 1.0 lbs (0.45 kg) for 25 cycles. No damage to the surface was observed.
• Humidity Test: The filters were exposed to a controlled temperature and humidity chamber set at 49±2°C and 95%–100% humidity for 24 hours under MIL-C-675C criteria. The film remained intact, with no peeling, scratches, or defects.
• Solvent and Cleaning Test: The test followed MIL-C-675C specifications and involved exposing the filter to acetone and alcohol at room temperature for at least 10 minutes each. After air drying and cleaning with a cotton cloth soaked in alcohol, the film showed no signs of peeling or scratches.
• Salt Spray Test: After 100 hours in a 35°C environment with a 5% saltwater concentration, the film showed no signs of damage, confirming high resistance to corrosion.

5. Standard Specifications

The Fluorescence Filter is manufactured to the following specifications:

6. General Applications and Examples

The Fluorescence Filter is widely used in a variety of scientific and industrial applications, particularly in microscopy and analytical instrumentation. Some key applications include:

• Fluorescence Microscopy: Used to isolate specific wavelengths in fluorescence microscopy, ensuring high-quality images of fluorescent specimens.
• Fluorescence Analyzer Equipment: Essential for use in fluorescence detection equipment, where precise wavelength isolation is critical for accurate measurements.
• Biotechnology and Analytical Applications: Crucial in biotechnology research, clinical chemistry, and spectroscopic analysis, where specific wavelengths need to be isolated for accurate results.
• GFP FRET Imaging: The filter is designed for imaging systems that utilize GFP FRET (Fluorescence Resonance Energy Transfer), ensuring that only the relevant wavelengths are transmitted.
• Photon Detection and Quantum Dot Imaging: Ideal for applications that require photon detection and imaging of quantum dots, where narrow wavelength isolation is crucial.

These filters provide essential capabilities for systems requiring high precision and high signal-to-noise ratios, making them a valuable component in advanced optical and analytical systems.

7. SOT Strengths and Capabilities

Shape Optics Technologies (SOT) is a leading provider of high-precision optical filters and components, with expertise in custom solutions for various applications. With years of experience in the field, SOT offers fluorescence filters that deliver superior performance, high transmission, and precise wavelength isolation. We utilize the latest manufacturing technologies to ensure our products meet the highest quality standards and provide our clients with the most reliable optical solutions for their applications. Whether you require standard or customized filters, SOT’s team is dedicated to meeting your needs with exceptional service and performance.

8. Standard Products

The following Fluorescence Filters are available as standard products:

Customization options are available upon request. Contact us for more information or to request a quote.