Time-Resolved Fluorescent Filters are engineered for applications that require precise temporal discrimination of fluorescence signals. In time-resolved techniques, separating short-lived background fluorescence from longer-lived emission is critical to achieving accurate, high-contrast results.
These filters are widely used in fluorescence lifetime imaging microscopy (FLIM), time-resolved fluorescence spectroscopy, immunoassays, and molecular diagnostics. SOT’s advanced multilayer dielectric coatings deliver high transmission in the passband and strong optical density outside the band, ensuring clean temporal and spectral separation.
Key Features
- Optimized for Time-Resolved Fluorescence (TRF) – High signal-to-noise ratio for accurate detection of fast fluorescence decay
- Advanced Coating Technology – High transmission, steep spectral edges, and excellent out-of-band blocking
- High Sensitivity – Enables detection of weak and short-lived fluorescence signals
- Customizable Designs – CWL, FWHM, optical density, size, and coating stack tailored to your system
- Durable & Reliable – Stable optical performance under demanding laboratory and diagnostic conditions
Coating Parameters
| Parameter | Value |
|---|---|
| Transmittance | T ≥ 90% (Customization is available upon request) |
| Center Wavelength (CWL) | Excitation: 365 nm, Emission: 610/615 nm (selection based on customer’s requirements) |
| Bandwidth (FWHM) | 20 ± 2 nm, 30 ± 2 nm (selection based on customer’s requirements) |
| Optical Density (OD) | OD4, OD5, OD6 (selection based on customer’s requirements) |
| Note | Product dimensions and other optical parameters can be customized. |
Applications
- Time-Resolved Fluorescence Spectroscopy – Accurate decay-time measurements
- FLIM (Fluorescence Lifetime Imaging Microscopy) – Molecular interaction and metabolic imaging
- Time-Resolved Immunoassays – Improved sensitivity by suppressing background fluorescence
- FRET Analysis – Detection of dynamic molecular interactions
- Molecular Biology & Biotechnology – Biomarker and protein interaction studies
- Medical Diagnostics – High-precision fluorescence-based diagnostic assays
Need a custom time-resolved fluorescence filter?
We offer matching dichroic mirrors and optical lenses. For more information, please feel free to contact us.