1. Features
- High transmission and steep cutoff depth for optimal performance.
- Designed to minimize crosstalk between adjacent channels, ensuring superior signal-to-noise ratio (SNR) for PCR experiments.
- Broad applicability in real-time fluorescent quantitative PCR (RT-qPCR) and digital PCR (dPCR).
- Customizable filter solutions available to meet specific application needs.
- Manufactured with advanced coating processes for durability and reliability.
2. General Description
Shape Optics Technologies Pte Ltd (SOT) specializes in high-performance Fluorescent PCR Filters, designed for critical applications in quantitative real-time PCR (RT-qPCR) and digital PCR (dPCR). These filters are engineered to offer high transmittance, minimal crosstalk, and a sharp cutoff, which significantly improves the signal-to-noise ratio (SNR) in PCR experiments. By optimizing the center wavelength (CWL) and full width at half maximum (FWHM), the filters efficiently separate the emission spectra of commonly used fluorophores. This level of precision ensures reliable and accurate results, even in complex multi-channel PCR assays. In addition, SOT offers customizable options to meet specific customer requirements, making them an ideal choice for diverse PCR applications.
Our filters undergo rigorous quality control and are compliant with military-grade adhesion, temperature, abrasion, humidity, and solvent resistance standards, ensuring high performance in demanding environments.
3. Spectrum Transmission Curves
Our standard Fluorescent PCR filters are tailored to meet the needs of a wide range of PCR applications. For customers with unique requirements, we offer the ability to customize filters based on specific wavelength ranges and spectral characteristics. Upon request, SOT can deliver customized solutions to suit any specialized applications in PCR-based research.
4. Coating Quality
The coating quality of our Fluorescent PCR filters is subjected to rigorous tests to ensure superior durability and reliability:
- Adhesion Test: Compliant with MIL-C-675C military standards, this test involves applying tape meeting LT-90 specifications onto the lens film. After applying and removing the tape vertically three times, no blistering or peeling occurs.
- Temperature Test: The filters are tested at extreme temperatures of -62±1℃ and 71±1℃, as per MIL-C-675C specifications. After two hours at room temperature (16℃–32℃), the adhesion is checked again, with no film detachment observed.
- Abrasion Resistance Test: Following MIL-C-675C and CCC-C-440 standards, this test involves applying a minimum force of 1.0 lbs (0.45 kg) using a gauze tester. The test is repeated 25 times without any surface damage to the film.
- Humidity Test: Filters are placed in a temperature and humidity chamber set to 49±2℃ and 95–100% humidity for 24 hours. The film remains intact with no defects, peeling, or scratches.
- Solvent and Cleaning Testing: Filters are exposed to acetone and alcohol at room temperature (16℃–32℃) for 10 minutes each. After cleaning, no peeling or scratches are observed on the film surface.
- Salt Spray Test: After 100 hours in a 35°C environment with a 5% saltwater concentration, the film remains undamaged, demonstrating excellent resistance to environmental factors.
5. Standard Specifications
| Model | CWL (nm) | FWHM (nm) | Transmittance | Optical Density | Dimensions |
| BP430/24 | 430±2 | 24±2 | Tave≥90% | OD≥3@635-800 | 3mm×3mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | OD≥3@635-800 | 4mm×4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 6mm×6mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 12mm×12mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 18mm×18mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP555/20 | 555±2 | 22±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
6. General Applications and Examples
Fluorescent PCR filters from Shape Optics Technologies are essential for a wide range of applications in life sciences and molecular biology:
- Real-time PCR: The filters are optimized for real-time quantitative PCR (RT-qPCR), ensuring accurate measurements of gene expression and other molecular assays.
- Digital PCR: Ideal for use in digital PCR systems, these filters enhance the sensitivity and specificity of detection by providing optimal spectral separation.
- Multiplex PCR: With minimal crosstalk, the filters enable the simultaneous detection of multiple targets, enhancing the throughput and efficiency of PCR-based diagnostics and research.
- Genomics: Used in genotyping, DNA sequencing, and other genomic research, ensuring high-performance fluorescence detection.
- Cellular Research: Facilitating the study of cellular processes and protein interactions, with filters designed to work with various fluorescent dyes.
- Diagnostics and Detection Systems: These filters are crucial in various diagnostic applications, ensuring that PCR-based systems deliver precise and reliable results.
7. SOT Strengths and Capabilities
Shape Optics Technologies Pte Ltd (SOT) has established itself as a leading provider of high-quality optical filters for a variety of scientific applications. With advanced coating technologies and a strong commitment to quality control, SOT’s filters ensure optimal performance under the most demanding experimental conditions. Their expertise in providing tailored solutions ensures that SOT can meet the specific needs of researchers and laboratories. The company’s technical capabilities include extensive customization options, providing the flexibility to adapt filters for any unique application. SOT’s commitment to precision, innovation, and customer satisfaction sets it apart as a trusted partner in the life sciences industry.
8. Standard Products
Fluorescent PCR Filter Product List
| Model | CWL (nm) | FWHM (nm) | Transmittance | Optical Density | Dimensions |
| BP430/24 | 430±2 | 24±2 | Tave≥90% | OD≥3@635-800 | 3mm×3mm |
| BP470/30 | 470±2 | 30±2 | Tave≥90% | OD≥3@635-800 | 4mm×4mm |
| BP480/20 | 480±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 6mm×6mm |
| BP485/20 | 485±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 12mm×12mm |
| BP520/20 | 520±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 18mm×18mm |
| BP525/20 | 525±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP530/20 | 530±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP555/20 | 555±2 | 22±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP565/20 | 565±2 | 22±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP570/20 | 570±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP585/20 | 585±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP595/20 | 595±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP612/20 | 612±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP620/20 | 620±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP630/20 | 630±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP655/20 | 655±2 | 22±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP660/40 | 660±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP670/20 | 670±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP682/15 | 682±2 | 15±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP715/20 | 715±2 | 20±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP725/40 | 725±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP725/60 | 725±2 | 60±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
| BP800/60 | 800±2 | 60±2 | Tave≥90% | OD≥3@635-800 | 25mm×25mm |
Additional Filters
| Model | AOI | Transmittance | Wavelength (nm) | Reflectivity | Dimensions |
| DM450 | 45° | Tave≥95% | 465-680 | R≥98% | 1(8)mm×8mm |
| DM550 | 45° | Tave≥95% | 515-700 | R≥98% | 2(11)mm×11mm |
| DM560 | 45° | Tave≥95% | 560-700 | R≥98% | 4(12)mm×10mm |
| DM590 | 45° | Tave≥95% | 575-700 | R≥98% | 5(16)mm×13mm |
| DM600 | 45° | Tave≥95% | 603-700 | R≥98% | 6(36)mm×25mm |
| DM635 | 45° | Tave≥95% | 622-700 | R≥98% | 7(50)mm×40mm |
| DM647 | 45° | Tave≥95% | 650-700 | R≥98% | 8(56)mm×40mm |
| DM700 | 45° | Tave≥95% | 665-700 | R≥98% | 9(71)mm×56mm |
| DM770 | 45° | Tave≥95% | 715-800 | R≥98% | 10(80)mm×80mm |
| DM800 | 45° | Tave≥95% | 780-900 | R≥98% | 11(88)mm×90mm |
