Fluorescent PCR Filters are purpose-built for high-precision fluorescence detection in RT-qPCR and dPCR systems. By carefully optimizing center wavelength (CWL) and bandwidth (FWHM), these filters deliver excellent channel isolation for commonly used fluorophores, significantly improving assay accuracy and repeatability—especially in multi-color and multiplex PCR.
Manufactured with advanced dielectric coating processes, SOT’s PCR filters provide high transmittance, sharp spectral edges, and robust environmental durability. Standard models cover a wide fluorophore range from blue to near-IR, while custom designs ensure seamless integration with specific light sources, detectors, and instrument geometries.
Key Features
- High Transmission & Steep Cut-off: Maximizes fluorescence throughput while sharply suppressing out-of-band noise for accurate quantification.
- Ultra-Low Crosstalk: Optimized spectral separation minimizes channel overlap, delivering superior signal-to-noise ratio (SNR) in multiplex PCR assays.
- RT-qPCR & dPCR Ready: Engineered for real-time quantitative PCR and digital PCR platforms across life-science and diagnostic workflows.
- Custom Spectral Design: CWL, FWHM, optical density, angle of incidence, and size can be tailored to specific dyes, detectors, and optical layouts.
- Proven Durability: Advanced multilayer coatings ensure long-term stability under thermal, humidity, abrasion, and solvent exposure.
Coating Parameters
| Parameter | Specification |
|---|---|
| Transmittance | Tavg ≥ 90% |
| Wavelength (CWL) | 400 nm – 850 nm (available options) |
| Bandwidth (FWHM) | 10 nm – 60 nm (available options) |
| Optical Density (OD) | ODabs ≥ 5; ODavg ≥ 6 @ 350–800 nm; OD ≥ 3 @ 800–1050 nm |
| Dimension | Standard: 6 × 6 mm; Diameter options: 6 mm, 12 mm, 25 mm (Aluminum oxide rings can be added as needed) |
| Thickness | 1.0 mm, 1.1 mm |
| Note | Product dimensions and other optical parameters can be customized |
Typical PCR Filter Set
| Fluorochrome | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| Atto 425 | BP430/24 | BP480/30 | DM450 |
| FAM / SYBR Green | BP470/30 | BP520/20 | DM505 |
| HEX / VIC / JOE | BP530/20 | BP565/22 | DM550 |
| CY3 / TAMRA | BP545/20 | BP585/20 | DM565 |
| ROX / Texas Red | BP570/20 | BP612/20 | DM590 |
| Atto 633 | BP620/20 | BP655/20 | DM635 |
| CY5 / Quasar 670 | BP630/20 | BP675/20 | DM647 |
| CY5.5 / Quasar 705 | BP675/20 | BP725/30 | DM700 |
| CY7 | BP725/30 | BP800/30 | DM770 |
Common Technical Parameters of PCR Filters
| Model | Wavelength (CWL, nm) | Bandwidth (FWHM, nm) | Model | Wavelength (CWL, nm) | Bandwidth (FWHM, nm) |
|---|---|---|---|---|---|
| BP430/24 | 430 ±2 | 24 ±2 | BP615/40 | 615 ±2 | 40 ±2 |
| BP470/30 | 470 ±2 | 30 ±2 | BP620/20 | 620 ±2 | 20 ±2 |
| BP480/20 | 480 ±2 | 20 ±2 | BP624/40 | 624 ±2 | 40 ±2 |
| BP480/30 | 480 ±2 | 30 ±2 | BP630/20 | 630 ±2 | 20 ±2 |
| BP485/20 | 485 ±2 | 20 ±2 | BP655/20 | 655 ±2 | 20 ±2 |
| BP520/20 | 520 ±2 | 20 ±2 | BP665/20 | 665 ±2 | 20 ±2 |
| BP523/20 | 523 ±2 | 20 ±2 | BP675/20 | 675 ±2 | 20 ±2 |
| BP525/20 | 525 ±2 | 20 ±2 | BP680/20 | 680 ±2 | 20 ±2 |
| BP530/20 | 530 ±2 | 20 ±2 | BP682/15 | 682 ±2 | 15 ±2 |
| BP545/20 | 545 ±2 | 20 ±2 | BP694/40 | 694 ±2 | 40 ±2 |
| BP565/20 | 565 ±2 | 22 ±2 | BP715/70 | 715 ±2 | 70 ±2 |
| BP570/20 | 570 ±2 | 20 ±2 | BP725/30 | 725 ±2 | 30 ±2 |
| BP580/20 | 580 ±2 | 20 ±2 | BP725/40 | 725 ±2 | 40 ±2 |
| BP585/20 | 585 ±2 | 20 ±2 | BP800/30 | 800 ±2 | 30 ±2 |
| BP612/20 | 612 ±2 | 20 ±2 | BP800/60 | 800 ±2 | 60 ±2 |
Common Technical Parameters of Dichroic Mirrors
| Model | Incidence Angle | Transmittance | Transmission Range (nm) | Reflectance | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM450 | 45° | Tavg ≥ 95% | 465–680 | R ≥ 95% | 380–445 |
| DM505 | 45° | Tavg ≥ 95% | 515–700 | R ≥ 95% | 450–490 |
| DM550 | 45° | Tavg ≥ 95% | 560–700 | R ≥ 95% | 450–538 |
| DM565 | 45° | Tavg ≥ 95% | 575–700 | R ≥ 95% | 450–555 |
| DM590 | 45° | Tavg ≥ 95% | 605–700 | R ≥ 95% | 450–580 |
| DM600 | 45° | Tavg ≥ 95% | 615–700 | R ≥ 95% | 450–590 |
| DM635 | 45° | Tavg ≥ 95% | 650–700 | R ≥ 95% | 450–630 |
| DM647 | 45° | Tavg ≥ 95% | 665–700 | R ≥ 95% | 450–640 |
| DM650 | 45° | Tavg ≥ 95% | 670–700 | R ≥ 95% | 450–645 |
| DM655 | 45° | Tavg ≥ 95% | 675–700 | R ≥ 95% | 450–650 |
| DM700 | 45° | Tavg ≥ 95% | 715–800 | R ≥ 95% | 450–685 |
| DM770 | 45° | Tavg ≥ 95% | 780–900 | R ≥ 95% | 600–755 |
| DM500(SP) | 45° | Tavg ≥ 95% | 440–485 | R ≥ 95% | 515–700 |
| DM550(SP) | 45° | Tavg ≥ 95% | 480–530 | R ≥ 95% | 555–700 |
| DM565(SP) | 45° | Tavg ≥ 95% | 480–550 | R ≥ 95% | 580–700 |
| DM600(SP) | 45° | Tavg ≥ 95% | 480–580 | R ≥ 95% | 620–700 |
| DM650(SP) | 45° | Tavg ≥ 95% | 480–635 | R ≥ 95% | 670–800 |
| DM690(SP) | 45° | Tavg ≥ 95% | 480–675 | R ≥ 95% | 710–800 |
Typical Applications
- Real-Time qPCR (RT-qPCR): Accurate gene expression and quantification
- Digital PCR (dPCR): High-specificity, low-noise fluorescence detection
- Multiplex PCR: Reliable multi-channel analysis with minimal spectral overlap
- Genomics & Molecular Diagnostics: Genotyping, mutation detection, and sequencing support
- Clinical & Research Instruments: Consistent performance in diagnostic and analytical systems
Need help selecting the right PCR filter set?
Share your fluorophores, detector type, and optical layout—SOT can optimize CWL, FWHM, and blocking to maximize SNR for your PCR system.