1. Features
- High transmittance and steep cutoff characteristics for accurate fluorescence imaging.
- Filters are designed for a variety of typical fluorophores, ensuring high sensitivity and resolution.
- Advanced coating process that provides enhanced durability and performance.
- Wide selection of standard models with customizable options to meet unique application requirements.
- Suitable for a range of microscopy applications, providing clear, high-quality fluorescence images.
2. General Description
Shape Optics Technologies Pte Ltd (SOT) offers a comprehensive range of Fluorescence Microscope Filters, which are manufactured using advanced coating processes to meet the stringent requirements of fluorescence microscopy. These filters are designed to provide optimal sensitivity and high-resolution fluorescence imaging by minimizing optical distortion and maximizing light transmittance. SOT’s filters cover the emission spectra of various fluorophores and are carefully optimized to minimize spectral overlap between excitation and emission wavelengths, thus enhancing the quality and clarity of the images. The filters come with a range of standard specifications and can also be customized to suit specific microscopy systems or research applications. These filters are durable and offer long-term performance in demanding experimental environments.
3. Spectrum Transmission Curves
Our Fluorescence Microscope filters are available with different spectral characteristics tailored to common fluorophores. For customers with specialized needs, we offer the option to customize the filters based on specific requirements. Customization can be made based on wavelength ranges, optical density, and transmittance levels to optimize for particular applications.
4. Coating Quality
The coating quality of our Fluorescence Microscope filters undergoes rigorous testing to meet military and industry standards:
- Adhesion Test: Compliant with MIL-C-675C military standards, the adhesion test involves applying tape meeting LT-90 specifications to the lens film, which is then removed vertically. After three repetitions, no blistering or peeling occurs.
- Temperature Test: The filters are exposed to temperatures of -62±1℃ and 71±1℃ as per MIL-C-675C standards. The adhesion test is repeated at room temperature (16℃–32℃) after two hours, confirming no detachment of the film.
- Abrasion Resistance Test: Per MIL-C-675C and CCC-C-440 standards, the abrasion resistance test is performed by applying a minimum force of 1.0 lbs (0.45 kg) with a gauze tester, repeated 25 times without damaging the film surface.
- Humidity Test: Filters are placed in a controlled temperature and humidity chamber (49±2℃ and 95%-100% humidity for 24 hours), with the film remaining intact, showing no defects such as peeling or scratching.
- Solvent and Cleaning Testing: In line with MIL-C-675C specifications, the filters are exposed to acetone and alcohol at room temperature (16℃–32℃) for 10 minutes each. After air drying and cleaning with a cotton cloth soaked in alcohol, no peeling or scratches are observed on the film.
- Salt Spray Test: After 100 hours in a 35°C environment with 5% saltwater, the filters remain undamaged, demonstrating exceptional resistance to environmental factors.
5. Standard Specifications
| Model | CWL (nm) | FWHM (nm) | Transmittance | Optical Density | Dimensions |
| BP375/28 | 375±2 | 28±2 | Tave≥85% | OD≥3@635-800 | 3mm×3mm |
| BP430/24 | 430±2 | 24±2 | Tave≥90% | OD≥3@635-800 | 5mm×5mm |
| BP460/55 | 460±2 | 55±2 | Tave≥90% | OD≥3@635-800 | 5mm×5mm |
| BP470/40 | 470±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 6mm×6mm |
| BP480/30 | 480±2 | 30±2 | Tave≥90% | OD≥3@635-800 | 6mm×6mm |
| BP530/40 | 530±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 7mm×7mm |
| BP535/40 | 535±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 8mm×8mm |
| BP605/55 | 605±2 | 55±2 | Tave≥90% | OD≥3@635-800 | 9mm×9mm |
| BP624/40 | 624±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 10mm×10mm |
| BP694/40 | 694±2 | 40±2 | Tave≥90% | OD≥3@635-800 | 11mm×11mm |
6. General Applications and Examples
Fluorescence Microscope filters from Shape Optics Technologies are widely used in biological and medical research, providing critical support for high-quality fluorescence imaging across numerous applications:
- Fluorescence Microscopy: Used in basic and advanced fluorescence microscopy techniques to capture detailed, high-resolution images of biological samples and tissues.
- Cell Biology and Histology: Ideal for studies requiring high-resolution imaging of cell structures, cellular processes, and molecular interactions.
- Immunofluorescence: Used to detect specific proteins or antigens tagged with fluorescent markers.
- Live Cell Imaging: Supports dynamic imaging of live cells, helping researchers study real-time processes at the molecular level.
- Molecular Biology: Essential for imaging and analyzing DNA, RNA, and protein expressions in samples using various fluorophores.
- Diagnostic Imaging: Used in clinical settings for precise fluorescence-based diagnostic imaging techniques.
7. SOT Strengths and Capabilities
Shape Optics Technologies Pte Ltd (SOT) is a leading manufacturer of high-quality optical filters, known for its expertise in providing custom solutions tailored to meet specific research and diagnostic needs. With state-of-the-art manufacturing processes and a commitment to quality control, SOT’s filters deliver exceptional performance across a wide range of applications. The company is dedicated to helping researchers and clinicians achieve the highest quality results in fluorescence imaging, providing filters that offer excellent spectral characteristics, long-lasting durability, and precise optical performance. SOT’s ability to customize filters to exact specifications and deliver them promptly is a testament to its commitment to customer satisfaction.
8. Standard Products
| Fluorophore | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
| DAPI | BP375/28 | BP460/55 | DM415 |
| Atto425/ECFP | BP430/24 | BP480/30 | DM450 |
| FITC/EGFP | BP470/40 | BP535/40 | DM505 |
| TRITC/PE | BP530/40 | BP535/40 | DM505 |
| CY5 | BP624/40 | BP694/40 | DM660 |
Customization Options
SOT offers the flexibility to customize Fluorescence Microscope Filters based on specific wavelength requirements, optical densities, and transmittance levels to optimize performance for your unique application. Please contact us for further details.
