Fluorescence Microscope Filters are manufactured using advanced thin-film coating processes to meet the stringent requirements of fluorescence microscopy. These filters are optimized to deliver high sensitivity, steep spectral transitions, and minimal spectral overlap between excitation and emission bands—critical for producing clear, high-contrast fluorescence images.
Covering the emission spectra of a wide range of fluorophores, SOT’s filters support both standard and advanced microscopy techniques. Robust construction and environmentally qualified coatings ensure long-term stability and consistent optical performance, even in demanding laboratory environments. Custom solutions are available to match specific microscope platforms, detectors, and research needs.
Key Features
- High Transmittance & Steep Cut-off: Optimized spectral edges reduce crosstalk and improve contrast for accurate fluorescence imaging.
- Fluorophore-Optimized Designs: Engineered for common fluorophores (DAPI, FITC/EGFP, TRITC/PE, Cy5, etc.) to maximize sensitivity and resolution.
- Advanced Coating Technology: Multilayer dielectric coatings deliver excellent durability, stability, and long service life.
- Wide Standard Selection & Customization: Broad range of standard bandpass options with tailored CWL, FWHM, OD, and sizes available.
- Microscopy-Ready Performance: Designed for demanding fluorescence microscopy workflows, from routine imaging to advanced research.
Coating Parameters
| Parameter | Value |
|---|---|
| Transmittance | T ≥ 90%, T ≥ 95% (Customized) |
| Wavelength (CWL) | 350-850 nm (Customization based on the customer’s requirements) |
| Bandwidth (FWHM) | 20–80 nm (Customization based on the customer’s requirements) |
| Optical Density (OD) | ODavg ≥ 6, ODabs ≥ 5 |
| Dimension | Conventional Dia 25 × 3.5 mm, Dia 25 × 5.0 mm (mounted) |
| Thickness | 1.0 mm, 1.1 mm |
| Note | Product dimensions and other optical parameters can be customized. |
Typical Fluorescence Microscope Filter Set
| Fluorescent Group | Excitation Filter (nm) | Emission Filter (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| DAPI/Alexa Fluor 350™ | BP375/28 | BP460/55 | DM415 |
| Atto 425/ECFP/Amycan | BP430/24 | BP480/30 | DM450 |
| FITC/EGFP/Alexa Fluor 488™ | BP470/40 | BP535/40 | DM505 |
| CY3™/TRITC/TAMRA | BP480/30 | BP535/40 | DM505 |
| ROX/mCherry/Alexa Fluor 594™ | BP530/40 | BP605/55 | DM565 |
| CY5™/Alexa Fluor 647™ | BP624/40 | BP694/40 | DM660 |
Common Fluorescence Microscope Filters
| Model | Wavelength (CWL, nm) | Bandwidth (FWHM, nm) | Model | Wavelength (CWL, nm) | Bandwidth (FWHM, nm) |
|---|---|---|---|---|---|
| BP375/28 | 375 ±2 | 28 ±2 | BP535/40 | 535 ±2 | 40 ±2 |
| BP430/24 | 430 ±2 | 24 ±2 | BP560/40 | 560 ±2 | 40 ±2 |
| BP460/55 | 460 ±2 | 55 ±2 | BP605/55 | 605 ±2 | 55 ±2 |
| BP470/40 | 470 ±2 | 40 ±2 | BP635/60 | 635 ±2 | 60 ±2 |
| BP480/30 | 480 ±2 | 30 ±2 | BP624/40 | 624 ±2 | 40 ±2 |
| BP530/40 | 530 ±2 | 40 ±2 | BP694/40 | 694 ±2 | 40 ±2 |
Common Fluorescence Microscope Dichroic Mirrors
| Model | Transmission Range (nm) | Reflection Range (nm) | Model | Transmission Range (nm) | Reflection Range (nm) |
|---|---|---|---|---|---|
| DM415 | 435–700 | 340–400 | DM600 | 615–700 | 450–590 |
| DM450 | 465–680 | 380–445 | DM605 | 620–750 | 450–595 |
| DM505 | 515–700 | 450–490 | DM655 | 670–750 | 450–645 |
| DM565 | 575–700 | 450–555 | DM660 | 675–750 | 450–600 |
Applications
- Fluorescence Microscopy – High-contrast imaging for biological samples
- Cell Biology & Histology – Detailed visualization of cellular structures
- Immunofluorescence – Accurate detection of labeled proteins and antigens
- Live-Cell Imaging – Real-time observation with minimal spectral interference
- Molecular Biology – DNA, RNA, and protein expression studies
- Clinical & Diagnostic Imaging – Reliable fluorescence-based diagnostics
Need a custom fluorescence filter set?
Share your fluorophore, excitation source, detector, and microscope platform—our engineers will optimize the spectral design for your application.