Fluorescence Microscope Filters are manufactured using advanced thin-film coating processes to meet the stringent requirements of fluorescence microscopy. These filters are optimized to deliver high sensitivity, steep spectral transitions, and minimal spectral overlap between excitation and emission bands—critical for producing clear, high-contrast fluorescence images.
Covering the emission spectra of a wide range of fluorophores, SOT’s filters support both standard and advanced microscopy techniques. Robust construction and environmentally qualified coatings ensure long-term stability and consistent optical performance, even in demanding laboratory environments. Custom solutions are available to match specific microscope platforms, detectors, and research needs.
Key Features
- High Transmittance & Steep Cut-off: Optimized spectral edges reduce crosstalk and improve contrast for accurate fluorescence imaging.
- Fluorophore-Optimized Designs: Engineered for common fluorophores (DAPI, FITC/EGFP, TRITC/PE, Cy5, etc.) to maximize sensitivity and resolution.
- Advanced Coating Technology: Multilayer dielectric coatings deliver excellent durability, stability, and long service life.
- Wide Standard Selection & Customization: Broad range of standard bandpass options with tailored CWL, FWHM, OD, and sizes available.
- Microscopy-Ready Performance: Designed for demanding fluorescence microscopy workflows, from routine imaging to advanced research.
Spectral Performance
A comprehensive selection of excitation and emission bandpass filters is available, each tuned to common fluorophores. For specialized applications, SOT provides custom spectral designs based on required wavelength ranges, optical density, and transmission targets to optimize image quality.
Coating Quality & Reliability
All fluorescence microscope filters undergo rigorous qualification:
- Adhesion: MIL-C-675C compliant (no blistering or peeling)
- Thermal Stability: −62 °C to +71 °C cycling
- Abrasion Resistance: MIL-C-675C / CCC-C-440 compliant
- Humidity Resistance: 95–100% RH at 49 °C
- Solvent Resistance: Stable after acetone & alcohol exposure
- Salt Spray: 100 h at 35 °C, 5% saline
Standard Specifications
| Model | CWL (nm) | FWHM (nm) | Transmittance | Optical Density | Size |
|---|---|---|---|---|---|
| BP375/28 | 375 ± 2 | 28 ± 2 | ≥85% | OD ≥ 3 @ 635–800 | 3 × 3 mm |
| BP430/24 | 430 ± 2 | 24 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 5 × 5 mm |
| BP460/55 | 460 ± 2 | 55 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 5 × 5 mm |
| BP470/40 | 470 ± 2 | 40 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 6 × 6 mm |
| BP480/30 | 480 ± 2 | 30 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 6 × 6 mm |
| BP530/40 | 530 ± 2 | 40 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 7 × 7 mm |
| BP535/40 | 535 ± 2 | 40 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 8 × 8 mm |
| BP605/55 | 605 ± 2 | 55 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 9 × 9 mm |
| BP624/40 | 624 ± 2 | 40 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 10 × 10 mm |
| BP694/40 | 694 ± 2 | 40 ± 2 | ≥90% | OD ≥ 3 @ 635–800 | 11 × 11 mm |
Typical Fluorophore Filter Sets
| Fluorophore | Excitation Filter | Emission Filter | Dichroic Mirror |
|---|---|---|---|
| DAPI | BP375/28 | BP460/55 | DM415 |
| Atto425 / ECFP | BP430/24 | BP480/30 | DM450 |
| FITC / EGFP | BP470/40 | BP535/40 | DM505 |
| TRITC / PE | BP530/40 | BP535/40 | DM505 |
| Cy5 | BP624/40 | BP694/40 | DM660 |
Applications
- Fluorescence Microscopy – High-contrast imaging for biological samples
- Cell Biology & Histology – Detailed visualization of cellular structures
- Immunofluorescence – Accurate detection of labeled proteins and antigens
- Live-Cell Imaging – Real-time observation with minimal spectral interference
- Molecular Biology – DNA, RNA, and protein expression studies
- Clinical & Diagnostic Imaging – Reliable fluorescence-based diagnostics
Need a custom fluorescence filter set?
Share your fluorophore, excitation source, detector, and microscope platform—our engineers will optimize the spectral design for your application.