Filters for Flow Cytometry are engineered to maximize measurement accuracy in cell analysis and sorting systems. By precisely controlling excitation and emission wavelengths, these filters improve fluorescence purity, reduce background noise, and increase measurement repeatability.
Flow cytometry is a cornerstone technology in cell biology, immunology, oncology, and clinical diagnostics, where accurate detection of fluorescence intensity and wavelength is critical. SOT’s filters utilize advanced multilayer dielectric coatings to deliver high transmission, strong optical density, and excellent spectral separation, ensuring reliable performance across a wide range of fluorophores and laser sources.
Key Features
- High Signal-to-Noise Ratio (SNR) – Effectively suppress stray light and spectral cross-talk, enhancing fluorescence signal clarity
- Advanced Optical Coatings – High transmission, steep cut-off edges, and excellent out-of-band blocking for accurate measurements
- Wide Wavelength Compatibility – Supports multiple laser lines and fluorophores commonly used in flow cytometry
- Customizable Solutions – CWL, FWHM, optical density, size, and coating designs tailored to your instrument
- Durable & Reliable – Qualified for long-term stability in demanding analytical and clinical environments
Spectral Performance
SOT provides both standard and custom spectrum designs, including bandpass, long-pass, short-pass, and dichroic filters. Transmission curves can be optimized for specific fluorophores, laser lines, and detector sensitivities to maximize system performance.
Coating Quality & Reliability
All Flow Cytometry Filters are tested to stringent international standards:
- Adhesion (MIL-C-675C) – No blistering or peeling after repeated tape tests
- Thermal Cycling – Stable from −62 °C to +71 °C
- Abrasion Resistance – MIL-C-675C / CCC-C-440 compliant (25 cycles @ 1.0 lb)
- Humidity Resistance – 95–100% RH at 49 °C for 24 h without defects
- Solvent Resistance – Stable after acetone and alcohol exposure
- Salt Spray Test – 100 h at 35 °C, 5% salt solution
Standard Specifications
| Model | CWL (nm) | Transmittance | FWHM (nm) | Optical Density | Dimensions |
|---|---|---|---|---|---|
| BP365/30 | 365 ± 2 | Tave ≥ 80% | 30 ± 2 | OD ≥ 5 @ 200–800 | Custom |
| BP365/30 | 365 ± 2 | Tave ≥ 90% | 30 ± 2 | OD ≥ 3 @ 200–800 | Custom |
| BP610/20 | 610 ± 2 | Tave ≥ 90% | 20 ± 2 | OD ≥ 4 @ 200–800 | Custom |
| BP610/30 | 610 ± 2 | Tave ≥ 90% | 30 ± 2 | OD ≥ 4 @ 200–800 | Custom |
| BP612/20 | 612 ± 2 | Tave ≥ 90% | 20 ± 2 | OD ≥ 4 @ 200–800 | Custom |
| BP612/30 | 612 ± 2 | Tave ≥ 90% | 30 ± 2 | OD ≥ 4 @ 200–800 | Custom |
| BP615/20 | 615 ± 2 | Tave ≥ 90% | 20 ± 2 | OD ≥ 4 @ 200–800 | Custom |
| BP615/30 | 615 ± 2 | Tave ≥ 90% | 30 ± 2 | OD ≥ 4 @ 200–800 | Custom |
Typical Flow Cytometry Filter Sets
| Fluorophore | Excitation Filter | Emission Filter | Dichroic Mirror |
|---|---|---|---|
| FITC | BP365/30 | BP460/55 | DM415 |
| PE | BP365/30 | BP480/30 | DM450 |
| APC | BP610/20 | BP635/20 | DM500 |
| PE-Cy7 | BP610/30 | BP710/40 | DM555 |
Applications
- Cell Sorting & Phenotyping – Accurate discrimination of cell populations
- Immunophenotyping – Reliable detection of antibody-labeled markers
- Cell Cycle & Apoptosis Analysis – Clear separation of fluorescence signals
- Clinical Diagnostics – Hematology, immunology, and disease monitoring
- Environmental & Microbial Studies – Detection of microorganisms in water or soil
- Genomics & Proteomics – Single-cell DNA and protein expression analysis
Need a custom flow cytometry filter set?
Share your laser lines, fluorophores, bandwidth, OD, and mechanical constraints—our engineers will design the optimal solution for your system.