Energy matching biochemical filter
  • Custom & Biochemical Filters

Energy Matching Biochemical Filters | High-Accuracy Optics

Energy Matching Biochemical Filters from Shape Optics Technologies Pte Ltd are engineered for high-accuracy biochemical and spectroscopic measurements. By precisely matching the spectral energy of the illumination source, detection channel, and sensor, these filters deliver excellent measurement accuracy, repeatability, and consistency.

This energy-matching approach is critical in complex biochemical assays where overlapping spectra can introduce errors. SOT’s advanced multilayer dielectric coatings ensure high cut-off depth, minimal inter-channel interference, and stable performance, making these filters ideal for biochemical analyzers, enzyme assays, and clinical diagnostic instruments.

 

Key Features

  • Advanced Coating Technology – High signal-to-noise ratio with sharp spectral edges for accurate absorbance detection
  • Optimized Energy Matching – Precisely matches the wavelength energy of light source, channel, and detector for superior linearity
  • High Cut-off Depth – Steep transitions minimize crosstalk and stray light interference
  • Customizable Designs – CWL, FWHM, optical density, size, and coating stack tailored to your system
  • Durable & Reliable – Long-term stability for demanding biochemical and clinical environments

 

Spectral Performance

Standard and custom spectrum transmission curves are available. Filters can be engineered to match exact system wavelengths and optical density requirements, ensuring optimal compatibility with your analyzer or detection platform.

energy matching biochemical filter transmission curve

 

Coating Quality & Reliability

All Energy Matching Biochemical Filters are qualified to stringent standards:

  • Adhesion (MIL-C-675C) – No blistering or peeling after repeated tape tests
  • Thermal Cycling – Stable from −62 °C to +71 °C
  • Abrasion Resistance – MIL-C-675C / CCC-C-440 compliant
  • Humidity Resistance – 95–100% RH at 49 °C for 24 h
  • Solvent Resistance – Stable after acetone and alcohol exposure
  • Salt Spray Test – 100 h at 35 °C, 5% saline

 

Standard Specifications 

Model CWL (nm) FWHM (nm) Transmittance Optical Density Size
NBP340 340 ± 2 10 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP405 405 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP450 450 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP492 492 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP505 505 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP510 510 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP546 546 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP578 578 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP600 600 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP630 630 ± 2 8 ± 2 Per request OD ≥ 4 @ 200–1100 Custom
NBP750 750 ± 2 8 ± 2 Per request OD ≥ 6 @ 200–1100 Custom

 

Typical Filter Sets 

Fluorophore Excitation Filter Emission Filter Dichroic Mirror
DAPI NBP340 NBP460 DM415
Atto425 / ECFP NBP405 NBP480 DM450
FITC / EGFP NBP470 NBP535 DM505
TRITC / PE NBP530 NBP535 DM505
Cy5 NBP624 NBP694 DM660

 

Applications

  • Biochemical Analyzers – High-accuracy absorbance and concentration measurements
  • Enzyme Assays – Improved linearity and repeatability
  • Spectroscopic Analysis – Clean spectral separation with minimal noise
  • Medical Diagnostics – Reliable enzyme and biomarker detection
  • Molecular Biology – DNA, RNA, and protein quantification
  • Environmental Testing – Precise optical measurements in monitoring systems

 

Need a custom energy-matching filter?
Share your light source, detector, target wavelength, bandwidth, and OD requirements—our engineers will design the optimal solution for your system.