Filters for flow cytometry

1. Features

• High Signal-to-Noise Ratio: Designed to purify light sources and enhance signal clarity, these filters optimize flow cytometry analysis by minimizing interference.
• Advanced Coating Technology: Utilizing cutting-edge coatings, these filters provide high transmission rates and excellent reflectivity for accurate measurements.
• Wide Wavelength Range: Suitable for analyzing various cells or particles, these filters support multiple laser wavelengths commonly used in flow cytometry.
• Customized Solutions: Filters can be tailored according to the specific requirements of the flow cytometry setup, including wavelength ranges and optical densities.
• Durable and Reliable: Manufactured for long-term stability, ensuring reliable performance under rigorous testing conditions in analytical instruments.

2. General Description

Shape Optics Technologies Pte Ltd (SOT) offers a comprehensive range of Filters for Flow Cytometry designed to improve the accuracy and precision of cell analysis and sorting. These filters utilize advanced coating technologies to provide exceptional transmission, high cut-off depth, and high reflectivity, making them ideal for flow cytometry applications.

Flow cytometry, an essential tool in cell biology and clinical diagnostics, relies on the accurate measurement of light emitted from fluorescently labeled cells or particles. SOT’s filters are specially designed to optimize these measurements by ensuring the purity of the light source, resulting in clearer signals and more reliable results.

These filters are used in flow cytometers to analyze various cell and particle characteristics, such as size, complexity, and fluorescence, making them essential for applications in medical diagnostics, research, and clinical testing. With customizable options, SOT provides solutions tailored to specific systems, ensuring that each filter meets the precise needs of the experiment.

3. Spectrum Transmission Curves

For specialized applications, SOT offers customized filter solutions. Filters can be tailored to specific wavelength ranges and optical densities, ensuring that your flow cytometry setup provides the best results. Contact us for details on customization.

4. Coating Quality

The Flow Cytometry Filters undergo stringent testing to meet industry standards:

• Adhesion Test: Compliant with MIL-C-675C military standards, involving the application of tape (LT-90 specification) to the lens film, followed by vertical removal. No blistering or peeling is observed after three repetitions.
• Temperature Test: Filters are subjected to temperatures of -62±1℃ and 71±1℃ as per MIL-C-675C specifications. No film detachment occurs after exposure and re-testing at room temperature (16℃–32℃) for two hours.
• Abrasion Resistance Test: Conforming to MIL-C-675C and CCC-C-440 standards, a gauze tester applies a minimum force of 1.0 lbs (0.45 kg), repeated 25 times, with no damage to the film surface.
• Humidity Test: The filters are exposed to a controlled temperature and humidity chamber set to 49±2℃ and 95%-100% humidity for 24 hours. The film remains intact without peeling or scratching.
• Solvent and Cleaning Testing: The filters are exposed to acetone and alcohol for at least 10 minutes at room temperature (16℃–32℃). After air drying and cleaning with alcohol, no peeling or scratches are observed.
• Salt Spray Test: Filters are tested in a 35°C environment with 5% saltwater for 100 hours, with no signs of damage.

5. Standard Specifications

6. General Applications and Examples

SOT’s Flow Cytometry Filters are essential in a variety of applications, including:

1. Cell Sorting and Analysis: Flow cytometry allows researchers to study and sort cells based on their characteristics, such as size, shape, and fluorescence. These filters optimize light transmission, ensuring clear, accurate results.
2. Immunophenotyping: Used to identify specific cell populations based on cell surface markers, these filters enable accurate detection of fluorescence emitted by antibody-bound cells.
3. Cell Cycle Analysis: Filters help to distinguish cells at various stages of the cell cycle, making them critical for cancer research and other cell biology studies.
4. Medical Diagnostics: Employed in clinical flow cytometry for diagnostic testing, such as detecting blood cell abnormalities or monitoring immune system functions.
5. Environmental and Ecological Studies: Used in the analysis of water or soil samples to detect microbial populations based on specific markers.
6. Genomics and Proteomics: Essential for measuring DNA and protein expression in single-cell analysis, making them critical in molecular biology research.

7. SOT Strengths and Capabilities

Shape Optics Technologies Pte Ltd (SOT) is a leading manufacturer of high-quality optical filters for flow cytometry. With years of experience and advanced manufacturing technologies, SOT’s filters ensure optimal performance in critical laboratory and diagnostic applications. The company is committed to providing customized solutions to meet the exact specifications of each customer. SOT’s products are backed by rigorous quality control standards and exceptional customer service, ensuring reliability and consistency in every order.

8. Standard Products

Customization Options

SOT offers the flexibility to customize filters for flow cytometry according to your specific wavelength, optical density, and system requirements. Please contact us for more details on customization options.